Study on virus-specific cytotoxic T-lymphocyte responses in Chinese human immunodeficiency virus type 1 infection
- VernacularTitle:中国人类免疫缺陷病毒-1感染者细胞毒性T淋巴细胞特异性应答
- Author:
Song ZHAI
;
Wen-Zhen KANG
;
Xu YU
;
- Publication Type:Journal Article
- Keywords:
HIV-1;
Acquired Immunodeficiency Syndrome;
T-lymphocytes,cytotoxic;
ELISPOT
- From:
Chinese Journal of Infectious Diseases
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the features of HIV-1-specific Cytotoxic T-Lymphocyte (CTL)responses in Chinese.Methods The HIV-1-specific CTL responses were analyzed in an IFN-?ELISPOT assay by using a matrix system containing 820 overlapping peptides spanning the entire HIV-1 Clade B and C consensus sequence.Results The HIV-1-specific CTL response almost clus- tered in Gag and Nef across either HIV-1 Clade B(HIV-1 B)or HIV-1 Clade C(HIV-1 C),while oth- er proteins could also be recognized at different level.In comparison of the response between HIV-1 Clade B and Clade C,the magnitude and frequency was roughly identical with some difference found at single-peptide level.The most frequently recognized peptides of HIV-1 B were located in Nef,GPKEP- FRDYVDRFYKTLR(5/17,29.4%)and Gag,LWVYHTQGYFPDWQNY(5/17,29.4%),while the most frequently recognized peptide of HIV-1 C was located in Gag GPKEPFRDYVDRFFKTLR(6/17, 35.29%).Conclusions HIV-1-specific CTL responses clustered within HIV-1 Gag and Nef in Chinese. However,there was some difference between HIV-1 B and HIV-1 C at single-peptide level.
