Soluble Proteins SDS-PAGE of C.tubulosa(Schenk) R.Wight
- VernacularTitle:不同寄主的管花肉苁蓉可溶性蛋白的电泳分析
- Author:
Pei CHEN
;
Yuhai GUO
;
Baomin WANG
;
Zhixi ZHAI
;
- Publication Type:Journal Article
- Keywords:
C.tubulosa(Schenk) R.Wight;
soluble-protein;
SDS-PAGE;
identification
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify 5 specimens of C.tubulosa(Schenk) R.Wight on different parasitifers,and explore the relationship between electrophoretic fingerprint and identification on soluble-protein,meanwhile providing data for establishing electrophoresis pattern library of Chinese crude drugs.Methods: The electrophoretic characteristics of soluble-proteins from 5 specimens of C.tubulosa(Schenk) R.Wight on different parasitifers were studied with SDS-PAGE.Results: It indicated that reproducible and high resolvable electrophoresis patterns of soluble proteins of C.tubulosa(Schenk) R.Wight on different parasitifers by SDS-PAGE.Their degrees of polymorphism were 55.56%.Conclusion: Each specimen had its own unique band pattern distinguish from the others.The electrophoresis pattern of SDS-PAGE of soluble proteins can be regarded as protein fingerprints for identification of C.tubulosa(Schenk) R.Wight on different parasitifers.
