The influences of long-term cryopreservation on cell viability and lymphocyte phenotypes of peripheral blood HS/PC collections.
- VernacularTitle:长期深低温冷冻对外周血造血干与祖细胞采集物中细胞活性及淋巴细胞亚群的影响
- Author:
Lei JIANG
;
Jian OUYANG
;
Junhao CHEN
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
HS/PC;
Cryopreservation;
Viability;
Phenotype
- From:
Chinese Journal of Practical Internal Medicine
2006;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influences of long-term cryopreservation on nucleated cell viability,colony-form- ing units-granulocyte-macrophage(CFU-GM)-forming capability and Lymphocyte immune-phenotypes of pe- ripheral blood hematopoietic stem/progenitor cell(HS/PC)collections.Methods Fresh samples(n=12)were as control,frozen samples were divided into two groups:group 1(cryopreserved for 3-5 years,n=26)and group 2 (cryopreserved for 5-7 years,n=9).Flow cytometry(FCM)method based on 7 AAD or caspase-3staining was ap- plied to assess the degree of cell necrosis or apoptosis in cryopreserved and red-cell lytic collection samples.Lympho- cyte immune-phenotypes(CD3/CD4/CD8/CD19/CD16+56,CD4/CIM5RA/CIMSRO,CD8/CD28)were also de- tected by FCM.CFU-GM culture was done to study the hematopoietic activity.Results Percentages of necrosis and apoptosis cells of the control were both less than the groupl and group2(P
