The effect of tamoxifen(TAM)in the chemotherapy of hepatocellular carcinoma(HCC)and its mechanism.
- VernacularTitle:三苯氧胺通过抑制PKC活性增强阿霉素诱导的肝细胞凋亡作用
- Author:
Yu SUN
;
Yan WU
;
- Publication Type:Journal Article
- Keywords:
Tamoxifen(TAM);
Hepatocellular carcinoma;
Cell line;
PKC;
Apoptosis
- From:
Chinese Journal of Practical Internal Medicine
2006;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate The effect of TAM(2.5 uM)in the chemotherapy of HCC and its mechanism. Methods An adriamycin-resistant human HCC cell subline(Hep-3B/ADM)was established,through exposure to gradually increased concentration of ADM.Drug sensitivity was measured by MTT.Modulation of ADM-induced apop- tosis of Hep-3B/ADM cells was assessed by Flow cytometry(FCM).PKC activity was determined by applying compet- itive protein binding method.Results TAM(2.5uM )decreased the IC50 of ADM from 1.64?g/mL to 0.23?g/mL in the Hep-3B/ADM cells.TAM exhibited a 2-fold potentiation on apoptosis caused by ADM in Hep-3B/ADM cells.With TAM,PKC activity was lower than that in control groups from 30 seconds to 24 hours.But this effect of TAM could be reversed by the co-treatment of the cells with TPA.Conclusion TAM(2.5 uM)significantly en- hanced ADM-induced cytotoxicity.The mechanism is that TAM potentiates the apoptosis induced by ADM through pro- tein kinase C inhibition.
