Influence of Conditioned Medium of Rat Brain Microvascular Endothelial Cells on Activity of Cortical Neurons and Protective Effect of Tong Luo Jiu Nao Injection
- VernacularTitle:大鼠脑微血管内皮细胞条件培养液对皮层神经元活性的影响以及通络救脑注射液的干预作用
- Author:
Weihong LI
;
Xuemei QING
;
Qian HUA
;
Jinghong HU
;
Qingtao HONG
;
Xiaolei ZHU
;
Pengtao LI
;
- Publication Type:Journal Article
- Keywords:
brain microvascular endothelial cell;
conditioned medium;
cortical neuron;
Tong Luo Jiu Nao Injection
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence of conditioned medium of rat brain microvascular endothelial cells on the activity of cortical neurons and the protective effect of Tong Luo Jiu Nao Injection.Methods: We collected the conditioned media from 4 different cultured endothelial cell groups,which were normal endothelial cells,the normal ones and treated with Tong Luo Jiu Nao Injection,the injured ones damaged by simulated cerebral ischemia,as well as the injured ones and treated with Tong Luo Jiu Nao Injection,respectively(N-CM,NT-CM,I-CM,IT-CM).Then,the conditioned medium was added into the cultures of the normal neurons and the damage ones which are injured by simulated cerebral ischemia as well,respectively.The effect of each type of conditioned medium on the activities of neurons was determined through the measurement of MTT and the transduction rate of LDH.Results:(1) N-CM has no obvious effect on the normal neurons,but does show some protective effect on the injured ones by increasing its activity significantly;(2) I-CM could decrease the activity of the normal neurons as well as aggravate the damage on the injured ones,while this injury effect can be reversed remarkably by IT-CM.Conclusion: The paracrine secretion of the brain microvascular endothelial cells might be one of the vital mechanisms in cerebral ischemic injury,indicating that the brain microvascular endothelial cells could be the therapeutic targets of Chinese medicine,which are not able to permeate through the Blood-Brain Barrier.
