Study on the mitochondrial mass change in dexamethasone induced mouse thymocyte apoptosis
- VernacularTitle:地塞米松诱导小鼠胸腺细胞凋亡中线粒体质量的变化
- Author:
Tong WANG
;
Yao-Ying ZENG
;
Fei-Yue XING
;
Pei-Yan LIANG
;
- Publication Type:Journal Article
- Keywords:
thymocyte;
apoptosis;
mitochondrial mass;
NAO;
mitotraker green
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study on the mitochondrial mass change in mouse thymocyte apoptosis.Method Control and Dexamethasone(DEX) groups were set;at 6h,we studied mitochondrial mass changes by NAO and Mitotracker Green(MG) staining flowcytometry and detected mitochondrial membrane potential change with DiOC_6(3) staining flowcytometry.We also used Annexin V-PE/MG double staining flocytometry to examine the mitochondrial changes in apoptosis progress.Results NAO staining results showed that 1 ?mol?L~(-1) DEX stimulation reduced the cardiolipin content of thymocyte mitochondria(P
