Boron neutron capture inducing apoptosis in C6 glioma cells
- VernacularTitle:硼中子俘获法诱导C6胶质瘤细胞凋亡
- Author:
Xiang ZHANG
;
Ruifeng CAO
;
Xinbiao JIANG
;
Jianning ZHANG
;
Zhou FEI
;
Yan XIAO
;
Xiling WANG
;
Jingwen LIANG
;
- Publication Type:Journal Article
- Keywords:
Boron neutron capture therapy;
Cell line,tumor;
Cell apoptosis
- From:
Chinese Journal of Radiation Oncology
2005;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of boron neutron capture therapy(BNCT) in C6 glioma cell line.Methods C6 cells in exponential phase were divided into 6 groups: untreated control,(~(60)Co?)(4 Gy),~(60)Co? 8 Gy,nuclear reactor exposure without boronophenylalanine(BPA) 3 Gy,BNCT(4 Gy) and BNCT 8 Gy.Cellular morphological change was observed by an inverted microscope,light microscope,fluorescence microscope and electronic microscope.Flow cytometry was used to determine the percentage of apoptosis,necrosis and normal cells 48h after irradiation.Colony forming assay was used to calculate cell surviving fraction.Results Typical morphological changes of apoptosis were observed early after irradiation in BNCT group,with a significant increase in apoptotic rates was observed 48 h after irradiation with 63.2% and 88.3% for BNCT(4 Gy) and 8 Gy group,respectively(P
