Evaluation of Chinese cobra venoms in preventing the kidney from ischemia/reperfusion injury
- VernacularTitle:中华眼镜蛇毒抑制肾缺血再灌注损伤的实验研究
- Author:
Fu-Ping XUE
;
Rong-Shan LI
;
- Publication Type:Journal Article
- Keywords:
Cobra venoms;
Ischemia-reperfusion injury;
Apoptosis;
Complement
- From:
Chinese Journal of Nephrology
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the renal protection of Chinese cobra venoms (CCV) and its mechanism in renal ischemia/reperfusion (I/R). Methods Thirty-two rats were divided into four groups. 0.1% CCV was separately infused into abdominal cavity at 0.5 h, 24 h before reperfusion in group Ⅰ and Ⅱ . Group Ⅲ suffered from kidney I/R was served as pathological control. Group Ⅳ was sham operation group. BUN and Scr were measured before ischemia and 24 h after reperfusion. Complement C3 was observed at 0, 0.5, 2, 24 h after reperfusion. The kidney samples were examined by HE stain under light microscopy. Apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP-biotin in situ nick-end labeling(TUNEL). Results Significant histological damage, apoptosis of tubular cell and impaired renal function were found in group Ⅰ and Ⅲ.The above indexes decreased to a less extend in group Ⅱ (P
