Mitochondrial mechanisms of apoptosis induced by artesunate in human leukemia HL60 cells
- VernacularTitle:青蒿琥酯诱导人早幼粒白血病细胞HL60凋亡的线粒体机制
- Author:
Lei NIE
;
Yilong YIN
;
Shaofu YIN
;
- Publication Type:Journal Article
- Keywords:
artesunate;
HL60 cell lines;
apoptosis;
mitochondrial membrane potential;
cysteine containing aspartate 3
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore mitochondrial mechanisms of apoptosis induced by artesunate in human leukemia HL60 cells. METHODS: Human leukemia HL60 cell lines were treated by artesunate. The inhibitory effect on cell proliferation was assessed by MTT assay. Electron microscopy and DNA gel electrophoresis was applied to demonstrate the presence of apoptosis. The level of MMP, cell cycle were determined by flow cytometry (FCM). The activity of caspase 3 was observed by colorimetry. RESULTS: Art showed a dose dependent and time dependent manner on the growth of HL60 cells (P
