Effects of Ca~(2+) /CaM-dependent calcineurin signaling pathway on cardiomyocytes hypertrophy of rats induced by neuropeptide Y
- VernacularTitle:Ca~(2+)/CaM-CaN途径参与神经肽Y诱导的大鼠心肌细胞肥大
- Author:
Qi DONG
;
Minsheng CHEN
;
Shaohua HUANG
;
Xiaoyun LI
;
Yinghui LI
;
Shu ZHANG
;
Minsheng CHEN
;
Shaohua HUANG
;
Xiaoyun LI
;
Yinghui LI
;
Shu ZHANG
;
Zhenxiu LIU
;
- Publication Type:Journal Article
- Keywords:
neuropeptide Y;
calcineurin;
cardiomyocytes hypertrophy
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM : To investigate the effects of Ca 2+ /CaM dependent calcineurin(CaN) signaling pathway on cardiomyocytes hypertrophy of rat induced by neuropeptide Y(NPY). METHODS : Cardiomyocytes of neonatal Wistar rats were cultured with NPY of various concentrations (10,100 nmol?L -1 ). Cyclosporine A (CsA) was used to inhibit the activity of CaN. The methods of 3H Leu incorporation was used to assess protein synthesis rate in cardiomyocytes. Western blot and histochemistry were used to measure CaN protein expression and CaN activity in cardiomyocytes. RESULTS : 3 H Leu incorporation of cardiomyocytes were increased significantly by 100 nmol?L -1 NPY ( P
