Observation on Legionella pneumophila surviving and multipling in Acanthamoeba polyphaga in vitro
- VernacularTitle:嗜肺军团菌感染多噬棘阿米巴及其增殖的实验观察
- Author:
Jie SHEN
;
Qingwu JIANG
;
Qinxue LI
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Legionella pneumophila;
Acanthamoeba;
Coculture
- From:
Chinese Journal of Infectious Diseases
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the survival and multiplication of Legionella pneumophila cocultured with Acanthamoeba polyphaga for setting up the laboratory model of such infections. Methods With the ratio of 1∶1000, Acanthamoeba polyphaga and Legionella pneumophila were cocultured under laboratory condition. We investigated the intracellular growth, survival and multiplication of Legionella pneumophila in Acanthamoeba polyphaga in morphological way by using inverted microscopy,electron microscopy and Giemsa staining of the cover glass picking up from the flask. Results Giemsa staining of the cover glass from the culture flask showed that the vacuoles appeared after 8 h coculture, and became more and bigger with the time. After 24 h, Giemsa staining showed that the bacteria started to accumulate within amoeba. In the anaphase of the coculture, the infected amoeba lacked defined organelle structures and was filled with legionella. The electron microscope revealed the survival of legionella in vacuoles of both trophozoites and cysts, showing different phases of binary fission. Conclusions Legionella pneumophila can survive and multiply in Acanthamoeba polyphaga . The results indicated that live amoeba could be a reservoir host for Legionella pneumophila.
