Expression of wild type and variant estrogen receptors in human hepatocellular carcinoma
- VernacularTitle:雌激素受体在原发性肝癌中的表达与变异
- Author:
Bao-Cai XING
;
Jia-Hong WANG
;
Yi WANG
;
Chun-Yi HAO
;
Xin-Fu HUANG
;
Yu WANG
;
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Receptors;
estrogen;
Variation;
Reverse transcriptase polymerase chain reaction
- From:
Journal of Peking University(Health Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of wild type estrogen receptor(wER)and the ex-on-5 deleted ER(variant ER,vER)in human hepatocellular carcinoma(HCC)samples,and thereafteranalyze the possibility of HCC treatment by endocrine therapy.Methods:The mRNA expressions of wERand vER were analysed from 28 cases of HCC by RT-PCR.The expression of ER at the protein level wasdetected by immunohistochemistry(IHC).Results:IHC results showed that 39.3% of the HCC speci-mens expressed ER.The mRNA of wER was detected in 89.3%(25/28)of the HCC specimens whilethat of vER was detected in 96.4%(27/28).Twenty four out of 28 HCC cases(85.7%)expressedboth wER and vER.One out of 28 patients(3.5%)expressed only wER whereas 3 patients out of 28(10.7%)expressed vER only.Conclusion:Ninety six percent(27/28)of the HCC patients expressedvER,which suggests that the expression of vER is an important event in the development of HCC.
