Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of l_?,25(OH)_2 vitamin D_3 on the secretion of OPG protein in vitro
- VernacularTitle:人牙周膜细胞骨保护因子和破骨细胞分化因子蛋白的表达及1?,25(OH)_2维生素D_3的调节
- Author:
Ding ZHANG
;
Yan-Qi YANG
;
Xiao-Tong LI
;
Min-Kui FU
;
- Publication Type:Journal Article
- Keywords:
Periodontium;
Cells;
cultured;
Calcitriol;
Membrane glycoproteins;
Osteoclasts
- From:
Journal of Peking University(Health Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of osteoprotegerin(OPG)and receptor activator nuclearfactor kappa B ligand(RANKL)at protein level in human periodontal ligament cells(HPDLCs),and theeffect of 1?,25(OH)_2 vitamin D_3[1,25(OH)_2 vitD_3] on the secretion of OPG protein in vitro.Meth-ods:HPDLCs were harvested in vitro by sequential digestion with trypsin and collagenase.The expressionof RANKL in HPDLCs at protein level was tested by immunocyto-chemistry.Enzyme-linked immuno-adsordent assay(ELISA)was used to detect the OPG protein which was secreted into the culture mediumby HPDLCs cultured with and without 10~(-8) mol/L 1?,25(OH)_2 vitD_3 on the 0,2nd,4th,and 6th days,respectively.Results:RANKL protein was detected on the membrane and plasma of HPDLCs,and OPGprotein was secreted in the culture medium.The secretion of OPG protein was down-regulated by 10~(-8)mol/L 1?,25(OH)_2 vitD_3.Conclusion:HPDLCs have the bone metabolism system of OPG/RANKL,which works during the process of 1?,25(OH)_2 vitD_3 inducing HPDLCs.The conclusion has laid thegroundwork for the study on bone remodelling mechanisms of HPDLCs.
