The mechanism of TGF-?1-induced changes of plasmin system in mesangial cells
- VernacularTitle:转化生长因子?1诱导的系膜细胞纤维蛋白溶酶系统变化及其机制
- Author:
Zongpei JIANG
;
Xueqing YU
;
Xionghui CHEN
;
Niansheng YANG
;
- Publication Type:Journal Article
- Keywords:
Transforming growth factor ?;
Plasmin;
Plasminogen activator;
Plasminogen activator inhibitor;
Reactive oxygen species
- From:
Chinese Journal of Nephrology
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of ROS in TGF ?1 induced changes of plasmin system in rat mesangial cells. Methods Growth arrested and synchronized rat mesangial cells were stimulated by 2 ng/ml TGF ?1 for 12 h. In some experiments cells were pretreated with BSO for 24 h or with antioxidant NAC for 1h. Intracellular ROS production was visualized using a fluorescent dye.The mRNA expression of tPA, uPA and PAI 1 was measured by RT PCR and PAI 1 protein secreted into media by ELISA assay.The activities of plasmin, uPA, tPA were determined using a synthetic fluorometric substrate. Results TGF ?1 significantly increased intracellular ROS concentration. 2 ng/ml TGF ?1 significantly upregulated PAI 1 mRNA and protein expression by 1 9 fold(P
