Observations on immune responses in mice induced by co immunizations of DNA vaccine of HBcAg and plasmids encoding interleukin 12 and interleukin 18
- VernacularTitle:HBcAg核酸疫苗与白细胞介素表达质粒联合接种小鼠的免疫应答观察
- Author:
Zuhu HUANG
;
Xin WU
;
Jun CHEN
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Vaccines, DNA;
Hepatitis B core antigens;
Interleukin-12;
Interleukin-18;
Mice
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the specific immune responses in mice induced by co immunization of DNA vaccine of HBcAg and plasmids encoding interleukin 12 and interleukin 18. Methods The mice were divided into following groups: vector alone, DNA vaccine of HBcAg alone, DNA vaccine of HBcAg plus plasmid of interleukin 12, DNA vaccine of HBcAg plus plasmid of interleukin 18, and DNA vaccine of HBcAg plus plasmids of interleukin 12 and interleukin 18. The mice were immunized with above DNA constructs by intramuscular injections. The levels of anti HBc and its isotypes(IgG1,IgG2a) in sera, and the level of IFN ? in supernatant of spleno lymphocyte cultures were measured by ELISA methods. CTL acti vities of spleno lymphocyte were detected with LDH release assay. Results Mice in all groups except for vector alone were sera positive for anti HBc. Comparing with group of DNA vaccine of HBcAg alone, groups of DNA vaccine of HBcAg plus plasmid of interleukin 12, DNA vaccine of HBcAg plus plasmid of interleukin 18, and DNA vaccine of HBcAg plus plasmid of interleukin 12 and interleukin 18 showed much higher end point titers of anti HBc( P
