Effects of Angiotensin Ⅱ and valsartan on expression of AT_1/AT_2 and eNOS in human umbilical vein endothelial cells
- VernacularTitle:血管紧张素Ⅱ和缬沙坦对血管内皮细胞AT_1/AT_2及eNOS表达的调节作用
- Author:
Yan WANG
;
Peihua WANG
;
Daowen WANG
;
- Publication Type:Journal Article
- Keywords:
angiotensin Ⅱ;
valsartan;
endothelial cells;
receptor;
endothelial nitric oxide synthase
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of angiotensin Ⅱ (AngⅡ) and valsartan on the expression of AngⅡ type 1 receptor (AT 1), AngⅡ type 2 receptor (AT 2) and endothelial nitric oxide synthase (eNOS) and the synthesis of nitric oxide (NO) in human umbilical vein endothelial cells (HUVECs, ECV304 cells). METHODS: Human umbilical vein endothelial cells (HUVECs) were incubated by Ang Ⅱ or valsartan for various periods. The expression of AT 1/AT 2, eNOS and the production of NO were detected. Potential mechanism of the above effects was explored by using the relevant inhibitors of the signal molecules and antioxidants. RESULTS: Both AngⅡ and valsartan significantly inhibited the mRNA and the protein expression of AT 1 in HUVECs. The combination of the two drugs caused more significantly inhibitory effects. AngⅡ showed a slightly promotive effect at first and a significantly inhibitory effect afterward on the expression of eNOS and synthesis of NO in HUVECs. MAPK inhibitor reversed the promotive effect whereas valsartan and antioxidant vitamine C significantly reversed the inhibitory effect. CONCLUSION: Valsartan can downregulate the expression of AT 1 in HUVECs. Long time action of AngⅡcan significantly inhibit the expression of eNOS and synthesis of NO in endothelial cells, while valsartan can significantly reverse this inhibitory effect.
