Polymorphism in CD14 promoter region in Han population in Chongqing
- VernacularTitle:重庆地区汉族CD14启动子区多态性分析
- Author:
Kai FENG
;
Xing LIU
;
Jianxin JIANG
;
- Publication Type:Journal Article
- Keywords:
Touchdown PCR;
restriction fragment length polymorphism;
CD14
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of CD14C ( 260)T single nucleotide polymorphism (SNP) in the Han population in Chongqing and to test the reliability with this random sample in genetic research. Methods Single round Touchdown PCR (TD PCR) and restriction fragment length polymorphism (RFLP) method were used for genotyping in 110 people from the Han population in Chongqing. Results Frequencies of TT, CT, and CC genotypes were 43 6%, 39 1%, and 17 3%, respectively. The sample distribution was accorded with Hardy Weinberg principle. Conclusion CD14C( 260)T SNP was found in the Han population in Chongqing, and the distribution of alleles was significantly different from that in white population. The sample from an H W equilibrium population can be used in the association study of the systematic inflammatory reaction in the Han population in Chongqing.
