Protective mechanism of aprotinin in platelet activation by Fura-2/AM dual-wavelength method
- VernacularTitle:应用Fura-2/AM荧光双波长法研究抑肽酶对血小板活化的保护作用
- Author:
Jie CHEN
;
Guocai TAO
;
Huaiqiong LIU
;
- Publication Type:Journal Article
- Keywords:
aprotinin;
platelet;
calcium indicator;
cytosolic free calcium
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the possible mechanisms of aprotinin in the protection of platelets. Methods Cytosolic free calcium concentration ([Ca 2+ ]i) was determined in calcium fluorescent indicator Fura 2/AM loaded washed human platelets by using dual wavelength spectrofluorophotometer. The mean value of resting [Ca 2+ ]i and the changes of [Ca 2+ ]i response to thrombin and aprotinin were observed. Results In the presence of extracellular Ca 2+ at the dose of 1 mmol/L, the resting level of [Ca 2+ ]i in platelets of human was (151.840?28.719) nmol/L. Thrombin stimulated the rise in [Ca 2+ ]i in the presence of Ca 2+ at the dose of 1 mmol/L, and the effects were inhibited by aprotinin in a concentration dependent manner ( P
