Effects of serum of pregnancy-induced hypertension on umbilical vein endothelial cells
- VernacularTitle:妊高征血清对脐静脉内皮细胞影响的研究
- Author:
Li LI
;
Xiguang ZHU
;
Li YU
;
Ping YI
;
- Publication Type:Journal Article
- Keywords:
pregnancy induced hypertension;
endothelial cell;
nitric oxide;
malondialdehyde;
superoxide dismutase;
lactate dehydrogenase
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes in secretory functions of cultured umbilical vein endothelial cells injured by serum from pregnancy induced hypertension (PIH). Methods Endothelial cells isolated from the umbilical veins from moderate or severe PIH women (PIH group, n =10) and normal pregnant women (control group, n =8) were incubated at 37 ℃ in humidified air with 5% CO 2. Cell culture fluids were obtained at 3 and 4 d of primary culture. Contents of nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), lactate dehydrogenase (LDH ) in the cell culture fluids were measured. Results LDH activity and MDA contents in the cell culture fluids in PIH group were significantly higher than those in the control group ( P
