Changes of ND1, ND2 and mtTFA gene expressions in myocardial cells and intestinal epithelial cells of rats after hemorrhagic shock
- VernacularTitle:失血性休克大鼠心肌和肠上皮细胞ND1、ND2、mtTFA基因表达的变化
- Author:
Rui CHEN
;
Yousheng LIU
;
Shuiming WANG
;
- Publication Type:Journal Article
- Keywords:
hemorrhagic shock;
mitochondrial respiratory chain complex Ⅰ;
gene expression
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the transcription pattern of ND1, ND2 and mtTFA gene in the myocardial cells and intestinal epithelial cells of rats after hemorrhagic shock. Methods Total RNA of myocardial cells and intestinal epithelial cells in rats were extracted after hemorrhagic shock. ND1, ND2, and mtTFA gene transcription levels were measured by reverse transcription and polymerase chain reaction (RT PCR). Results During the period from 1 to 2 h after hemorrhagic shock, the ND1 gene transcription levels in myocardial cells in the hemorrhagic shock groups were higher than that in the normal control group, but the levels in intestinal epithelial cells were lower than that in the normal control group. The pattern for the changes of ND2 gene transcription in myocardial cells and intestinal epithelial cells was basically similar. Conclusion There might exist certain tissue differences in the changes of ND1 gene transcripts in myocardial cells and intestinal epithelial cells of rats with hypoxic and ischemic damage.
