Culture of outer root sheath cells of murine vibrissa follicles in vitro and expression of uPA/uPAR
- VernacularTitle:小鼠触须毛囊外根鞘细胞的体外培养及uPA和uPAR表达研究
- Author:
Qiangguo GAO
;
Gang FU
;
Tian YANG
;
- Publication Type:Journal Article
- Keywords:
hair follicle;
outer root sheath cell;
dermal sheath cell;
uPA/uPAR
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the cultivation of hain follicle outer root sheath (ORS) cells of murine vibrissa and to study their expression of uPA/uPAR. Methods The ORS cells of murine vibrissa were cultured by combination of enzyme digestion and tissue culture with the feeder layer of dermal sheath cells of the hair follicle. The cells and their uPA/uPAR expression were identified by immunocytochemistry (ICC). Results The feeder layer of DS was suitable for the culture of ORS cells. ORS cells expressed uPA/uPAR protein. Conclusion The DS of hair follicle is a better feeder layer for the growth of ORS cells. ORS cells have the ability to migrate and proliferate.
