Corticotropin-releasing hormone regulates corticotropin-releasing hormone mRNA expression through PKA signal pathway in rat hypothalamic slices in vitro
- VernacularTitle:CRH通过PKA途径调节大鼠下丘脑CRH表达的研究
- Author:
Lizhao CHEN
;
Minhui XU
;
Jihong ZHOU
;
- Publication Type:Journal Article
- Keywords:
stress;
hypothalamic slice in vitro;
corticotropin releasing hormone;
corticotropin releasing hormone receptor;
protein kinase A;
cAMP;
response element binding protein;
CP 154526;
H89;
rat
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the signal regulatory mechanism of expression of corticotropin releasing hormone (CRH) stimulated neurons of hypothalamic slices in rats in vitro . Methods Model of hypothalamic slices of rats was established. After CRH stimulatation of corticotropin releasing hormone type 1 receptor (CRH1R) of hypothalamic slices in rats in vitro , the changes of activity of protein kinase A (PKA) signal passway were observed by immunocytochemical method and Western blotting. The relationship between the changes and CRH mRNA expression was also observed. Results CRH could cause the remarkable increase in phosphorylated PKA, phosphorylated CREB, and CRH content in hypothalamic slices in rats. However, CPl54526 or H89 could have significant inhibitory effect on the synthesis of P PKA, P CREB, and CRH. Conclusion PKA signal passway can regulate the ultrashort positive feedback of CRH secretion in the rat hypothalamus in stress due to severe trauma.
