Effect of pentoxifylline on mesangial cells proliferation and expression of connective tissue growth factor cultured in high glucose
- VernacularTitle:己酮可可碱对高糖培养的系膜细胞增殖及结缔组织生长因子表达的作用
- Author:
Ting YE
;
Xiaocheng LIU
;
- Publication Type:Journal Article
- Keywords:
pentoxifylline;
connective tissue growth factor;
mesangial cell;
diabetic nephropathy
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of pentoxifylline(PTX) on rat mesangial cells(MCs) proliferation and the expression of connective tissue growth factor(CTGF) protein cultured in high glucose concentration. Methods The MCs were divided into 4 groups: ①normal glucose group(NG,5 mmol?L -1 D glucose),②high glucose group(HG, 30 mmol?L -1 D glucose),③HG+0 36 mmol?L -1 PTX,④HG+1 08 mmol?L -1 PTX. 72 hours later, the proliferation activity of mesangial cells was observed by MTT assay, the expression of CTGF protein was detected by Western blot. Results Compared with normal glucose group, high glucose significantly increased MCs proliferation activity and the expression of CTGF. PTX of different concentration suppressed the proliferation of the MCs and down regulated the expression of CTGF. Conclusion PTX can suppress the proliferation activity of MCs cultured in high glucose and decrease the expression of CTGF, which implies PTX probably decreases the accumulation of extracellular matrix(ECM) and slows the progression of glomerular hypertrophy and glomerulosclerosis in this way. Thus it demonstrates the protective effect of PTX in diabetic nephropathy(DN) from a new angle.
