Effect of a novel isoquinoline derivative,CPUC1 on the reversal of multidrug resistance in K562/A02 cells
- VernacularTitle:异喹啉类的新化合物CPUC1对K562/A02细胞多药耐药的逆转作用
- Author:
Yuanyuan HOU
;
Haojie ZHU
;
Ling HE
;
Guoqing LIU
;
- Publication Type:Journal Article
- Keywords:
multidrug resistance;
apoptosis;
isoquinoline;
CPUC1
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the reversal effect of CPUC1 on multidrug resistance in K562/A02 cells. Methods MTT assay was used to detect the cytotoxicity of vincristine. Intracellular accumulation of rhodamine123 was measured by flow cytometry to evaluate the function of P glycoprotein(Pgp). The apoptotic cells induced by vincristine were detected by flow cytometry using DNA content analysis and Annexin V/PI double staining. Results CPUC1 significantly reversed the resistance of K562/A02 cells to vincristine. CPUC1 increased intracellular accumulation of rhodamine123 in K562/A02 cells in a concentration dependent manner. CPUC1 increased the apoptosis induced by vincristine. Conclusion CPUC1 reversed multidrug resistance by inhibiting function of P gp in K562/A02 cells, suggesting that CPUC1 merits further study.
