Inhibition of proliferation and influence of Proto-oncogenes expression by matrine in C6 cell
- VernacularTitle:苦参碱对U251胶质瘤细胞的增殖抑制和原癌基因表达的影响
- Author:
Hui DENG
;
Huanmin LUO
;
Feng HUANG
;
Wen WENG
;
Peifen ZHANG
;
- Publication Type:Journal Article
- Keywords:
matrine;
U251 glioma cell;
cell cycle;
expression of proto-oncogene
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim This study was designed to investigate the inhibition of matrine on U251 glioma cell line and its mechanism. Methods MTT was used to measure the levels of the proliferation of U251 cultured with matrine in different concentrations.The effects of matrine on cell cycle of U251 were observed by FCM. The expression of proto oncogenes c myc was measured by RT PCR. Results The proliferation of U251 was obviously inhibited by matrine in a dose dependent manner. The inhibitory rate was (53 7?6 0)%,when cultured with matrine at 0 10 g?L -1 . The outcome of FCM showed that the proportion of G 0/G 1 phase cells were decreased. The proportion of S phase cells were reduced obviously,when cultured with matrine at 0 10 g?L -1 in 3 days.The outcome of RT PCR showed that the expression of proto onco gene C myc was notably decreased, when the dose of matrine was increased. Conclusion Matrine can inhibit the proliferation of U251 and inhibit the expression of proto onco gene C myc.
