Protective effects of huang lian jie du tang active fraction on ischemia injury in cerebral cortical neuronal cultures
- VernacularTitle:黄连解毒汤有效部位对培养大鼠皮层神经元缺糖缺氧损伤的保护作用
- Author:
Yan WU
;
Jianning SUN
;
Shaokun YU
;
- Publication Type:Journal Article
- Keywords:
huang lian jie du tang active fraction(HLJDTAF);
cerebral cortical neuron;
ischemia;
cell culture
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effects of huang lian jie du tang active fraction(HLJDTAF) on ischemia injury in cerebral cortical neuronal cultures. Methods The cortical neurons of rat fetal were cultured in vitro. The protective effects of HLJDTAF on ischemia injury were observed by treating cells with sodium dithionite in glucose free medium. Results HLJDTAF 610 mg?kg -1 increased the activity of neuron, 305 mg?kg -1 reduced the leakage rate of LDH; HLJDTAF 610, 305, 153 mg?kg -1 reduced the content of NO,MDA and increased the activity of SOD. Conclusion HLJDTAF protected the cerebral cortical neurons from ischemia injury by reducing the contents of NO and suppressing the generation of lipidperoxide.
