Protective effect and mechanisms of overexpressed PPAR? against fibrosis induced by high glucose in cultured mesangial cells
- VernacularTitle:过氧化物酶体增殖物活化受体?减少高糖诱导系膜细胞外基质的积聚及其机制
- Author:
Jing SUN
;
Ji MA
;
Yong GU
;
Shan-Yan LIN
;
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferator;
Transfection;
Signal pathway;
Mesangial cells
- From:
Chinese Journal of Nephrology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
0 05).Among them,WT but neither DN, Blank, nor Pio significantly ameliorated the increased concentrations of TGF-a1 and fibro nectin in culture medium induced by HG. Compared with DN and Blank, WT transfect ion significantly attenuated high glucose-caused elevation in c-fos, c-jun and p-ERK expression, reduction in Ⅰ-?B level, and incretio n in nucleus/cytosol ratio of NF-?B, while Pio demonstrated similarly signific ant changes only in p-ERK and NF-?B. No significant difference of GLUT-1 mRN A level was detected between HG groups. Conclusions Overexpressed PPAR?1 can su ppress increased ECM production from glomerular mesangial cells cultured in HG c ondition. Its inhibitory effects on activation of ERK/AP-1 and NF-?B pathways are involved. The further increase of PPAR?activity may have direct anti-fibr otic effect in diabetic kidney.
