Simultaneous determination of lactulose and mannitol excretion ratio in urine by high performance liquid chromatography
- VernacularTitle:高效液相色谱法检测尿乳果糖/甘露醇排出比值及成人正常值
- Author:
Fangnan LIU
;
Li TAN
;
Nan LUO
;
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Lactulose;
Mannitol;
Intestinal permeability
- From:
Parenteral & Enteral Nutrition
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Lactulose and mannitol excretion ratio in urine appears to be a marker to measure the intestinal permeability. Methods: A method has been developed for the simultaneous determination of lactulose and mannitol in urine by high performance liquid chromatography.After ultrafiltered and deionized,urine samples were analyzed with a lichrosher NH 2 column(4.6 mm?250 mm,i.d.,5 ?m),a mix of acetonitril water (72∶28,volume ratio) as mobile phase and refractive index detector. Results: The recoveries were 97.86%、 99.19% respectively and the relative standard deviation were 2.12、2.04.In 18 urine samples from healthy volunteers and patients who ingested the test solution containing 10 g lactulose and 5 g mannitol,the 6 h urinary excretion ratio of lactulose/mannitol was 0.0275?0.0113. Conclusions: It is suggested that lactulose and mannitol excretion ratio was anaccurate marker of intestinal permeability.
