Effects of survivin on radiation injury and its relation to Caspase-9/6,3 activity
- VernacularTitle:Survivin对辐射损伤的影响及与caspase-9/6、3的关系
- Author:
Yang GAO
;
Chengji LUO
;
Haidi LI
;
Yehua ZHENG
;
- Publication Type:Journal Article
- Keywords:
surivivin;
radiation;
apoptosis;
caspase;
antiapoptosis protein
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of survivin on radiation injury and its relation to caspase 9/6,3 activity. Methods ① IEC 6 cells were treated under hypoxic environment for 8 h and followed by reoxygenation for 24 and 48 h, and then the expression of survivin was identified by Western blotting. ② After treatment with 35 Gy 60 Co , the apoptosis rate in different groups was detected with FACS and the caspase 9/6,3 activity was tested using the caspase 9/6,3 assay kit. Results Hypoxic preconditioning could induce the expression of survivin in IEC 6 cells and survivin could decrease the caspase 3 activity, resulting in reduced apoptosis rate induced by radiation. Conclusion Survivin induced by hypoxic preconditioning can inhibit the apoptosis induced by radiation by reducing the caspase 3 activity.
