Comparison of the effects of ursodeoxycholic acid, dexamethasone, and S-adenomethionine on intrahepatic cholestasis induced by ethinylestradiol in pregnant rats
- VernacularTitle:熊脱氧胆酸、地塞米松、思美泰对抗孕大鼠肝内胆汁淤积症的比较研究
- Author:
Qing CHANG
;
Jingbo WANG
;
Yong CHEN
;
Changxu SHI
;
- Publication Type:Journal Article
- Keywords:
ursodeoxycholic acid;
dexamethasone;
S-adenomethionine;
intrahepatic cholestasis of pregnancy;
rat
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ursodeoxycholic acid (UDCA), dexamethasone (DEX), and S adenomethionine (SAMe) on the pregnant rats with intrahepatic cholestasis induced by ethinylestradiol (EE). Methods A total of 50 15 day pregnant Wistar rats were randomly divided into five groups: control group, EE group, UDCA group, DEX group, and SAMe group. In EE group, rats were subcutaneously injected with EE (2.5 mg?kg -1 ?d -1 ) for 5 d. In the control group, rats received subcutaneous injection of appropriate volume of propylene glycol (PG) for 5 d. In UDCA, SAMe, and DEX groups, rats were administered with the same dose of EE plus UDCA (100 mg?kg -1 ?d -1 ), SAMe (100 mg?kg -1 ?d -1 ), and DEX (1 mg?kg -1 ?d -1 ) for 5 d, respectively. The serum levels of aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatases (ALP), glycocholic acid (GC), and total bilirubin were measured after cesarean. Morphologic changes of the liver of pregnant rats and the growth and development of the fetal rats were observed. Results The serum levels of ALT, ALP, GC in rats of UDCA group were decreased significantly as compared with those in DEX group ( P
