Changes and significance of P~(53) , C-myc, Bax, and Bcl-2 in surviving HepG_2 cells under continuous stress of cisplatin
- VernacularTitle:顺铂持续压力下HepG_2存活细胞中P~(53)、C-myc、Bax、Bcl-2的变化及意义
- Author:
Weiqing CHEN
;
Wei SHEN
;
Dingming SHEN
;
- Publication Type:Journal Article
- Keywords:
HepG 2;
cisplatin;
Bcl-2;
Bax
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the dynamic changes of P 53 , C myc, Bax, Bcl 2, and NF ?B in surviving HepG 2 cells under the continuous stress of cisplatin for the investigation of the potential role in the resistance of HepG 2 cells to cisplatin. Methods The apoptosis of HepG 2 cells and changes of the apoptosis associated factors including P 53 , C myc, Bax, Bcl 2, and NF ?B in the surviving HepG 2 cells were detected by immunohistochemical and RT PCR assays. Results No obvious change of P 53 and C myc was found in the surviving HepG 2 cells under cisplatin stress, but persistent increase of Bcl 2 and decrease of Bax and a continuous increase of Bcl 2/Bax ratio were found. Markedly positive expression of NF ?B mRNA was found at 7 d after the stress of cisplatin. Conclusion The mechanisms of the surviving HepG 2 cells under continuous stress of cisplatin may be associated with the increase in Bcl 2 and decrease in Bax, specially the continuous increase in Bcl 2/Bax ratio, while the positive NF ?B mRNA expression may be associated with the later events after HepG 2 cell survive.
