Expression of glucose transporter 4 and cyclin kinase inhibitor p21 in cultured glomerular mesangial cells stimulated by high glucose
- VernacularTitle:高糖刺激肾小球系膜细胞葡萄糖转运蛋白4及p21的表达及其意义
- Author:
Songmin HUANG
;
Fei LIU
;
Wanxin TANG
;
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Glucose transporter 4;
Cyclin dependent kinase inhibitor p21;
Cellular hypertrophy
- From:
Chinese Journal of Nephrology
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the relationship between expressive level alteration of glucose transporter 4(GLUT 4) mRNA,cyclin kinase inhibitor p21 mRNA and glomerular mesangial cell(GMC) hypertrophy in cultured rat GMC.Methods Cultured rat 1097 GMCs were divided into high glucose group, mannitol group, different insulin concentration groups, high glucose plus different insulin concentration groups and control group. Semi quantity RT PCR and flow cytometery were used to detect GLUT 4mRNA and p21mRNA levels and GMC volume. Results A certain expression of GLUT 4mRNA and p21mRNA from GMC was found in control group. High glucose decreased GLUT 4mRNA level and increased p21mRNA level. Insulin up regulated GLUT 4mRNA expression in a dose dependent manner.The more p21mRNA expressed, the stronger forward scatter (FSC) was and the bigger GMC became. Conclusions High glucose can cause GMC hypertrophy. Up regulation of p21mRNA and down regulation of GLUT 4mRNA may be involved in GMC hypertrophy and glomerular hypertrophy in early diabetic nephropathy.
