Roles of reactive oxygen species in HepG_2 cell apoptosis induced by CDDP
- VernacularTitle:活性氧在顺铂致HepG_2凋亡中的作用
- Author:
Weiqing CHEN
;
Wei SHEN
;
Dingming SHEN
;
- Publication Type:Journal Article
- Keywords:
CDDP;
ROS;
apoptosis;
HepG 2
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of acivicin on HepG 2 cell apoptosis and the effects of acivcin with cis diaminedichloroplatinum (CDDP) on HepG 2 cell apoptosis and on production of intracellular ROS. Methods HepG 2 cells were treated with acivcin, CDDP, and acivicin combined with CDDP. The cytotoxicity was measured by MTT assay. Flow cytometry was used to detect the intracellular ROS, and the rate of apoptosis was determined by TUNEL assay. Results The concentration of acivicin to cause HepG 2 IC 50 was 1.4 mmol/L, and CDDP was 67 ?mol/L. Significant increases in intracellular ROS content were observed in HepG 2 cells 24 h after treatment with acivicin (1.4 mmol/L), CDDP (67 ?mol/L), or acivicin (1 4 mmol/L) plus CDDP (67 ?mol/L), especially with acivicin (1 4 mmol/L) plus CDDP (67 ?mol/L). HepG 2 cell apoptotic rates induced by acivicin (1 4 mmol/L) at 24, 48, and 72 h were (16 3?3 5), (27 9?4 3), (47 2?3 0), respectively, and a significant difference was observed compared to that of control group ( P
