Effects of polyclonal antibody to lipopolysaccharide binding protein on IL-10 and IL-18 mRNA expressions of alveolar macrophages in lipopolysaccharide-induced acute lung injury in rats
- VernacularTitle:LBP多抗对内毒素急性肺损伤大鼠肺泡巨噬细胞IL-10、IL-18基因表达的影响
- Author:
Deming ZHANG
;
Yongwang LI
;
Baoling MAO
;
Guisheng QIAN
;
- Publication Type:Journal Article
- Keywords:
lipopolysaccharide;
lipopolysaccharide binding protein;
acute lung injury;
alveolar macrophage
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of polyclonal antibody to lipopolysaccharide binding protein (pLBPab) on IL 10 and IL 18 mRNA expressions of alveolar macrophages in lipopolysaccharide LPS induced acute lung injury in rats. Methods A total of 40 male Wistar rats were randomly divided into 5 groups: normal control (A), LPS treated group (B), group of pLBPab preconditioning at 30 min before injection of LPS (C), group of treatment with LPS and pLBPab (D), and group of pLBPab preconditioning at 30 min after injection of LPS (E). mRNA expressions of IL 10 and IL 18 in the alveolar macrophages in each group were measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR). Results The IL 10 and IL 18 mRNA expressions were highly increased respectively in the alveolar macrophage (AM?) stimulated with single LPS, but they were significantly decreased in the AM? after stimulation with LPS + pLBPab, particularly stimulation with pLBPab 30 min before LPS injection. Conclusion pLBPab can decrease the mRNA expressions such as IL 10 and IL 18 by alveolar macrophages in acute lung injury in rats induced by LPS, and LBP antibody could be used to cure some diseases such as SIRS, acute lung injury, ARDS, and septic syndrome.
