Activation of p38 mitogen-activated protein kinase induced by tumor necrosis factor-? in human pulmonary microvascular endothelial cells
- VernacularTitle:肿瘤坏死因子-?激活人肺微血管内皮细胞p38丝裂原激活蛋白激酶信号通路
- Author:
Yong SONG
;
Yi SHI
;
Jieshou LI
;
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor-?;
Mitogen-activated protein kinase;
Endothelial cell;
Signal transduction
- From:
Chinese Journal of Respiratory and Critical Care Medicine
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of tumor necrosis factor ?(TNF ?) on p38 mitogen activated protein kinase (MAPK) activation in human pulmonary microvascular endothelial cells (HPMEC) in vitro Methods HPMEC was isolated and cultured in vitro Expression and activation of p38 MAPK were detected by western blot and immune complex kinase assay Results TNF ? induced phosphorylation and activation of p38 MAPK in HPMEC in a time/dose dependent manner which was suppressed by SB203580,a specific inhibitor of p38 MAPK Conclusion TNF ? plays a important role in activation of p38 MAPK and signal transduction in HPMEC
