Different mRNA expressions of calcium activated channels in normal and instable detrusors
- VernacularTitle:逼尿肌不稳定中钙激活钾/氯通道mRNA表达差异的实验研究
- Author:
Hang YANG
;
Bo SONG
;
Xiyu JIN
;
Xin YANG
;
- Publication Type:Journal Article
- Keywords:
detrusor instability;
calcium activated potassium channels;
calcium activated chloride channels;
RT PCR;
mRNA expression
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the different mRNA expressions of big and small conductance calcium activated potassium channels (Bkca and Skca), and calcium activated chloride channels (Clca) in normal and instable detrusors. Methods Model of bladder outlet obstruction (BOO) of female Wistar rats was prepared by ligating the proximal urethra in the perineum. Detrusor instability (DI) was confirmed by conscious cystometry. The mRNA was extracted from the detrusors of normal and DI rats for the detection of the expressions of Bkca, Skca2, Skca3, and Clca by RT PCR. The different channel expression between normal and instable detrusors was identified by gel imaging. Results The incidence of DI in BOO rats was 76.17%. Bladder capacity and the maximal detrusor pressure increased significantly ( P
