Effects of NDGA on the growth of a human malignant glioma cell line CHG-5
- VernacularTitle:去甲二氢愈创木酸对人恶性胶质瘤细胞系CHG-5生长的影响
- Author:
Chengping XU
;
Xiuwu BIAN
;
Rong ZHANG
;
Yusong YIN
;
- Publication Type:Journal Article
- Keywords:
nordihydroguaiaretic acid;
glioma;
cell cycle;
apoptosis;
xenograft
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the growth of a human malignant glioma cell line CHG 5 in vitro and in vivo . Methods Colorimetric MTT assay, flow cytometry, and light microscopy were used to investigate the proliferation in vitro , cell cycles, apoptosis of CHG 5 cells, and the growth of xenografted tumor in nude mice. Results NDGA significantly inhibited the proliferation of CHG 5 cells in vitro . Cells in G 0/G 1 phase increased, but cells in S, G 2/M phases decreased, and apoptotic cells increased significantly. After treatment of NDGA (50 mg/kg, intraperitoneally) at 5 d after the inoculation of tumor cells, the xenografted tumor volume reduced remarkably without causing significant toxic and side effects. Conclusion The inhibitory effect of NDGA on the growth of CHG 5 cells may be correlated with the regulation of cell cycles and induction of apoptosis.
