Typing of Acinetobacter baumannii isolated from patients in intensive care unit with IRS-PCR
- VernacularTitle:IRS-PCR对重症监护病房患者标本分离鲍曼不动杆菌的分型研究
- Author:
Qingling ZHANG
;
Yufu LIU
;
Minghua LIU
;
Xianyuan WANG
;
Weiling FU
;
- Publication Type:Journal Article
- Keywords:
biotyping;
genotype;
Acinetobacter baumannii;
polymerase chain reaction
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To type Acinetobacter baumannii using infrequent restriction site PCR (IRS PCR). Methods Strain specific electrophoretic patterns from PCR products by amplifying DNA sequences flanking infrequent restriction sites of 15 strains of Acinetobacter baumannii were compared with the results of biotyping and antimicrobial susceptibility. Results The 15 bacteria were divided into 5 gene types with IRS PCR, but 3 with biotyping, and 4 with antimicrobial susceptibility. Conclusion IRS PCR method for typing Acinetobacter baumannii is of strong sensitivity, high recognition, good repeatability, convenient operation, and wide range of application.
