Construction of bioartificial renal tubule assist device and determination of transport to creatinine,amino acids and sodium in vitro
- VernacularTitle:生物人工肾小管体外构建及对氨基酸、钠离子、肌酐转运功能的研究
- Author:
Xumin YING
;
Xiaoyun WANG
;
Xia SHEN
;
- Publication Type:Journal Article
- Keywords:
Biomedical technology;
Bioartificial renal tubule;
LLK PK1 cells;
Transport
- From:
Chinese Journal of Nephrology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the method of constructing bioartificial renal tubule assist device(RAD) and its transport function to creatinine,glucose,sodium and asparagine,theonine,serine,glutamic acid in vitro.Methods The cultured LLC PK1 cells were seeded into the inner space of the hemofilter(FH66)and the hemofilter was incubated for two weeks.Then the filtration in vitro was simulated to detect the function of the RAD.The transport rates of sodium,creatinine,glucose and four kinds of amino acids were measured.Meanwhile,the effects of oubaine and phlorizin on the transport rates of sodium,glucose and amino acids were determined and compared with no LLC PK1 device.Results The creatinine transport rate of RAD was significantly decreased as compared with control group(P
