Contribution of small GTPase Rho effector—Rock-Ⅰin different stages of rat renal interstitial fibrosis
- VernacularTitle:Rock-Ⅰ激酶在肾间质纤维化大鼠不同阶段肾组织中的激活及意义
- Author:
Hua SUN
;
Ronghua CHEN
;
Wenyan HUANG
;
Li FEI
;
Mei GUO
;
Xiaoqin PAN
;
- Publication Type:Journal Article
- Keywords:
Rock Ⅰkinase;
Ureteral obstruction;
Renal interstitial fibrosis
- From:
Chinese Journal of Nephrology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of Rock Ⅰand its functional activation in renal tissue from a rat UUO model, and to investigate its role in the progression of renal interstitial fibrosis(RIF). Methods Expression of RockI mRNA and protein were examined by RT PCR and Western blotting, respectively.The phosphorylation of MBS(binding subunit of myosin phosphatase)—a substrate of Rock Ⅰwas detected by Western blotting and defined,as the mark of functional activation of the kinase. Results (1)The expression of Rock ⅠmRNA was increased before the onset of RIF(the 3rd day after the experiment) (F=15 18,P
