Zinc finger protein gene A20 inhibits lipopolysaccharide-induced IL-8 expression of endothelial cells
- VernacularTitle:转染锌指蛋白基因A20抑制内毒素诱导的内皮细胞IL-8表达的研究
- Author:
Jianhong MI
;
Chuhong ZHU
;
Dajun YING
;
- Publication Type:Journal Article
- Keywords:
A20 gene;
interleukin 8;
lipopolysaccharide
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of zinc finger protein gene A20 on the inhibition of lipopolysaccharide (LPS) induced interleukin 8 (IL 8) expression in endothelial cells. Methods Plasmid pcDNA3.1EHA20 was transfected into human umbilical vein endothelial cells (HUVECs) by DOTAP method. The positive cell clones were screened with G418. The stable transfection and expression of A20 in HUVECs were determined by immunofluorescent analysis. IL 8 expression was detected by sandwich ELISA with double monoclonal antibodies. Results High expression of A20 gene in HUVECs transfected with pCDNA3.1EHA20 was confirmed by immunofluorescent analysis. IL 8 expression increased in LPS induced endothelial cells. A20 gene could inhibit more than 70% LPS induced IL 8 expression ( P
