Effects of somatostatin analogues on the cell cycle and the related gene expression in human cholangiocarcinoma cell line SK-ChA-1
- VernacularTitle:生长抑素类似物对胆管癌SK-ChA-1细胞株及其相关基因表达的影响
- Author:
Hua CHEN
;
Zhenping HE
;
Kuansheng MA
;
Zhong YANG
;
- Publication Type:Journal Article
- Keywords:
somatostatin;
human cholangiocarcinoma;
SK ChA 1 cells;
cell cycle;
gene expression
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of somatostatin analogue (SMS201 995, SMS) on the cell cycle of human cholangiocarcinoma SK ChA 1 cells and cell cycle regulated gene cyclinD1 and p16. Methods SK ChA 1 cells were synchronized by serum starvation. Cells at different phases were harvested at 48 h after SMS treatment of the synchronized SK ChA 1 cells. The distribution of cell cycles was analyzed by flow cytometry. The expression levels of cyclinD1 and p16 gene were detected by SABC immunohistochemistry and in situ hybidization. Results SMS could significantly inhibit the poorly serum synchronized SK ChA 1 cells to reenter the cell cycle from G 0/G 1 phase at 6 h and 12 h ( P
