Effects of anti-lipopolysaccharide egg yolk immunoglobulin on LPS in vitro
- VernacularTitle:抗内毒素IgY体外拮抗内毒素效应的实验研究
- Author:
Qiang LI
;
Yaping ZHANG
;
- Publication Type:Journal Article
- Keywords:
immunoglobulin Y;
lipopolysaccharide;
human umbilical vein endothelial cell;
lactic dehydrogenase;
fluorescein isothiocyanate lipopolysaccharide
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of anti lipopolysaccharide (LPS) immunoglobulin Y (IgY) on LPS in vitro . Methods The direct in vitro neutralization of different concentrations of IgY and LPS was conducted for the detection of the remained amount of LPS to observe the dose effect relationship. The primary cultured human umbilical vein endothelial cells (HUVECs) were cultured for 48-72 h after addition of certain concentrations of fluorescein isothiocyanate LPS (FITC LPS) and IgY. Changes of the fluorescent intensity in HUVECs were observed at different time points. After addition of certain concentrations of LPS and IgY for incubation with HUVECs for 4, 8, and 12 h, the changes of lactate dehydrogenase (LDH) activity were measured. Results The dose effect relationship was satisfactory when the ratio was 16︰1. At 4, 8, and 12 h, fluorescent intensity was somewhat strong in FITC LPS group, but weak in IgY group, especially at 8 and 12 h. The LDH activity in medium increased obviously after incubation for 8 h in LPS group ( P