PPAR? expression and its effects on the differentiation of mesenchymal stem cells induced by 5-Azacytidine in vitro
- VernacularTitle:5-氮杂胞苷诱导小鼠骨髓间充质干细胞分化过程中PPAR?表达及其作用
- Author:
Xianhe LIN
;
Longgui LI
;
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cell;
induction;
PPAR?
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of peroxisome proliferator activated receptor? (PPAR?) and its roles in the differentiation of mesenchymal stem cells (MSCs) induced by 5 Azacytidine. Methods 5 Azacytidine was used to induce the differentiation of primary cultured mesenchymal stem cells. The eukaryotic expression plasmid vector pEGFP N1 PPAR? was transfected into MSCs with lipotransfection method followed by G418 selection. The expression levels of PPAR? mRNA were detected by RT PCR. Immunohistochemistry and Western blot were employed to study the protein expression of PPAR?. Lipid droplets in the cells were stained with Oil Red O. Results No expression of PPAR? mRNA was found in undifferentiated MSCs. After induction by 5 Azacytidine, partial MSCs expressed PPAR? and differentiated into lipoblasts. MSCs, transfected with pEGFP N1 PPAR?2, differentiated into adipocytes. Conclusion 5 Azacytidine can induce the differentiation of MSCs into myoblasts and lipoblasts, which may be related to the expression of PPAR?.
