Effects of E-cadherin gene transfection on bladder cancer cell growth and invasion
- VernacularTitle:E-cadherin基因转染对膀胱癌细胞生长及浸润能力的影响
- Author:
Jinhong PAN
;
Xiyu JIN
;
Bo SONG
;
Enqing XIONG
;
- Publication Type:Journal Article
- Keywords:
transitional cell carcinoma of bladder;
growth;
infiltration;
gene transfection
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of E cadherin (ECD) on bladder cancer cell growth and invasion using gene transfection technique. Methods Human E cadherin gene was transfected into a highly invasive and metastatic bladder cancer cell line Ts3L. Cell growth curves, clone formation rate, in vitro tumor cell infiltrating ability, and cell cycle distribution were determined, respectively. Results Speed of bladder cancer cell growth decreased significantly to 35 7% after ECD gene transfection. Apoptotic cell rate in ECD transfected cells was markedly higher than that of the control cells. The percentage of superploid cells in Ts3L/ECD cells was significantly reduced than that in the control cells. Clone formation rate of ECD transfected cells, detected by soft agarose gel clone formation test, was significantly decreased as compared with that of the control cells ( P
