Effects of HLA-derived peptide on proliferation and heme oxygenase-1 activity of rat spleen cells
- VernacularTitle:HLA衍生肽对大鼠脾细胞增殖功能及HO-1活性的影响
- Author:
Shanhong YI
;
Bo SONG
;
Zehou WANG
;
Genfu ZHANG
;
Zhigang CUI
;
- Publication Type:Journal Article
- Keywords:
HLA derived peptide;
mixed lymphocyte reaction;
heme oxygenase 1
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunosuppression function of a novel HLA derived peptide, RDP1258, and its mechanisms. Methods A peptide derived from HLA, RDP1258, was chemically synthesized by artificial solid phase synthesis. Effects of the peptide on alloreactive cytotoxic activity of rat spleen cells and heme oxygenase 1 (HO 1) activity were observed using 3H TdR method and enzyme chemistry method, respectively. Results The synthetic HLA derived peptide could obviously inhibit the proliferation of rat spleen cells and mixed lymphocyte reaction, and reduce HO activity in a dose dependent manner in vitro . Conclusion RDP1258 can significantly inhibit the proliferation of rat spleen cells induced by mitogen and isoantigen possibly by means of affecting HO 1 activity.
