Growth hormone with glutamine accelerate remnant intestinal adaptation after massive small bowel resection in rat
- VernacularTitle:生长激素联合谷氨酰胺对促进残存小肠适应性代偿的研究
- Author:
Yuanxin LI
;
Xin ZHOU
;
Ning LI
;
Jieshou LI
;
- Publication Type:Journal Article
- Keywords:
Short bowel syndrome;
Growth hormone;
Glutamine;
Small intestine adaptation
- From:
Parenteral & Enteral Nutrition
1997;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: In order to investigate effect and mechanism of solely or combined treatment of glutamine (Gln) and growth hormone (GH) on remnant intestinal adaptation after massive small bowel resection, we observed the weight of the rats, and analyzed morphology, nutrients absorptive function and gene expression of the remnant intestine. Methods: All rats undertaken 85% small bowel resection and placement of intravenous catheter and gastric tube were randomly assigned to 4 groups :①glycine (SB); ②GH+ glycine (SB/GH); ③Gln (SB/Gln); ④Gln+GH (SB/GH/Gln). All rats received isocaloric, isonitrogenous and isovolumic nutrition support. All rats were weighed daily after operation. On the POD12, crypt depth, villus height and mucosa thickness of remnant small bowel were analyzed; the \[ 14 C\] glucose and \ palmitic acid absorptive test were in vivo assayed; plasma insulin like growth factor 1(IGF 1) was measured; IGF 1, sodium glucose cotransport 1(SGLT1) and intestinal fatty acid binding protein(I FABP) mRNA were semiquatified analyzed by RT PCR. Results: The body weight, plasma IGF 1, villous height and mucosal thickness,\[ 14 C\] glucose and \ palmitic acid absorption both in SB/GH group and SB/Gln group had significantly increased than those in SB group, while those in SB/GH/Gln were significantly higher than those in any other group. The mRNA expression of IGF 1, SGLT1 and I FABP in SB/GH and SB/GH/Gln groups were significantly higher than those in SB and SB/Gln groups. Conclusions: Combined treatment of GH and Gln can enhance the intestinal trophic effect after massive small bowel resection. Gln can enlarge the mucosal absorptive surface in remnant small bowel. GH can not only enlarge the mucosal absorptive surface, but also enhance absorptive functional gene expression of enterocyte via mediation by local expression of IGF 1 .
