Cellular mechanism of renal proximal tubular epithelial cell injury induced by aristolochic acid Ⅰ and aristololactam Ⅰ
- VernacularTitle:马兜铃酸Ⅰ及马兜铃内酰胺Ⅰ对肾小管上皮细胞损伤的差异
- Author:
Biao LI
;
Xiaomei LI
;
Cuiying ZHANG
;
Xuan WANG
;
Shaoqing CAI
;
- Publication Type:Journal Article
- Keywords:
Aristolochic acid;
Kidney tubules;
Epithelial cells;
Apoptosis;
Extracellular matrix
- From:
Journal of Peking University(Health Sciences)
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the cellular mechanism of renal proximal tubular epithelial cell(PTEC) injury induced by aristolochic acid Ⅰ (AA Ⅰ) and aristololactam Ⅰ (AL Ⅰ). Methods:Human PTEC cell line HK 2 was used as the subject. Cell apoptosis was evaluated by using FACS. Fibronectin (FN) and TGF ?1 levels were assayed in the supernatant from cultured HK 2 cells by ELISA. In the blocking study, anti TGF ?1 neutralizing antibody was used as an antagonist. The changes of FN level and apoptosis were compared. Results: After stimulation by 2.5 mg/L AA Ⅰ, HK 2 cells secreted TGF ?1 at hour 12 and FN at hour 36. HK 2 cell apoptosis was detected at hour 48. Anti TGF ?1 neutralizing antibody (5 mg/L) could suppress AA Ⅰ induced apoptosis by 63.7% ( P
