Culture of osteoblasts by serial explant culture and comparison of their characteristics
- VernacularTitle:连续植块培养原代成骨细胞及其特性的比较
- Author:
Zhiying WANG
;
Xing WANG
;
Junjun XIAO
;
Xiaomin DONG
;
Shucong MENG
;
Man ZHAO
;
- Publication Type:Journal Article
- Keywords:
Osteoblasts;
Cell culture/methods;
Immunohistochemistry
- From:
Journal of Peking University(Health Sciences)
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To Investigate a simple, economical and efficient method of primary osteoblast culture and compare their characteristics. Methods:Primary osteoblasts of 1 st -4 th series explant culture from calvarial bones of neonatal Sprague Dawley rats,were collected and osteoblasts shape, mitosis, proliferation, ALP activity and immunohistological expression of osteocalcin and BMP 2 observed. Results:No difference was found in cell shape, spread, conflunce, ALP activity as well as immunohistological stain of osteocalcin and BMP 2 of the osteoblasts, which had been harvested from the 1 st -4 th series explant cultures of Sprague Dawley rats. Conclusion:Series explant culture can harvest the same kind of osteoblasts as the explant culture, and more osteoblasts can be obtained at a single time. It saves money and time and is easy to manipulate.
