Effects of 17?-estrogen, testosterone, tamoxifen, and aminoglutethimide on the growth and proliferation of lung adenocarcinoma cell line A549
- VernacularTitle:17?-雌二醇睾酮三苯氧胺和氨鲁米特对肺腺癌细胞株A549生长增殖的影响
- Author:
Xiangdong ZHOU
;
Wenqin CAI
;
Lan XIAO
;
Jiqiang ZHANG
;
Zhong YANG
;
- Publication Type:Journal Article
- Keywords:
pulmonary carcinoma;
estradiol;
testosterone;
tamoxifen;
aromatase
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the aromatase protein expression in A549 cell and to investigate the effects of 17? estrogen (E 2), testosterone (T), estrogen receptor antagonist tamoxifen (TAM), and aromatase inhibitor DL aminoglutethimide (AMIN) on the growth and proliferation of A549 cells. Methods The expression of aromatase protein was determined by immunohistochemical methods. The changes of cell cycle and cell number before and after treatment with E 2, T, TAM, and AMIN were measured by flow cytometry and tetrazolium method (MTT). Results The aromatase protein was positively expressed in A549 cells. The aromatase inhibitor AMIN and 5?10 -7 mol/L TAM could inhibit the growth of A549 cells and block them in G 0/G 1 phase ( P
